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KMID : 0380020090240040393
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Korean Journal of Biotechnology and Bioengineering
2009 Volume.24 No. 4 p.393 ~ p.396
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Development of Protein Secretion System using Type III Secretion System of Salmonella
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Vo Dinh Le Tam
Hong Soon-Ho
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Abstract
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New protein secretion system was developed using Type III Secretion System of Salmonella. N-terminal region of SlrP and SptP effector proteins were fused with TliA and EstA-P lipases by overlapping PCR. Lipase activity of Salmonella with SptP-TliA fusion system increased by 2.6 fold compare with wild type Salmonella strain. This result showed that lipase secretion via the T3SS would be a useful protein secretion machinery.
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KEYWORD
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Type III Secretion System, Salmonella, secretion, lipase
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